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Objective:To evaluate the effect of Mongolian medicine Eerdun Wurile on postoperative cognitive function in elderly patients undergoing thoracoscopic lobectomy.Methods:Sixty elderly patients of either gender, aged ≥60 yr, of American Society of Anesthesiologists Physical Status classification Ⅱ or Ⅲ, with body mass index≤28 kg/m 2, with preoperative Mini-Mental State Examination (MMSE) score ≥27 points, undergoing thoracoscopic lobectomy under general anesthesia, were divided into 2 groups ( n=30 each) using the random number table method: control group (group C) and Eerdun Wurile group (group E). Eerdun Wurile 15 capsules per day were taken for 7 consecutive days starting from 3 days before surgery in group E, and placebo was given instead in group C. The peripheral venous blood samples were collected before medication at 3 days before surgery (T 1) and 24 and 72 h after surgery (T 2, 3) for determination of the concentrations of serum brain-derived neurotrophic factor (BDNF), interleukin-1beta (IL-1β), tumor necrosis factor-alpha (TNF-α) and Tau proteins by enzyme-linked immunosorbent assay. The patients′ cognitive function was assessed by MMSE at T 1 and 5 days after surgery (T 4), and the occurrence of cognitive decline (MMSE score < 27) was recorded. Results:Compared with group C, the serum BDNF concentration was significantly increased at T 2 and T 3, and the concentrations of IL-1β, TNF-α and Tau protein were decreased, the MMSE score was increased at T 4, and the incidence of cognitive decline was decreased in group E ( P<0.05). Conclusions:Mongolian medicine Eerdun Wurile can improve postoperative cognitive function in elderly patients undergoing thoracoscopic surgery, and the mechanism may be related to reduction of systemic inflammatory responses and promotion of nerve cell repair and regeneration.
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Objective To observe the effect of the Mongolian medicine on the apoptosis after spinal cord injury. Methods A total of 12 rats were divided into the sham group, the model group, and the like-3 treatment groups (Naru-3 group), 4 in each group. A rat model of spinal cord injury (SCI) was established by modified Allen's method. After 72 h, we sacrificed the rats, separated spinal cord cells, which were filled with 5×105 shop in every hole, training for subsequent testing after 24 h. The relative expression of caspase-3 was detected by RT-QPCR and western blot, and apoptosis was analyzed by flow cytometry. Results Compared to the model group, the expression of caspase-3 mRNA (2.177 ± 0.084 vs. 3.287 ± 0.098) and caspase-3 protine (1.063 ± 0.157 vs. 2.180 ± 0.147) in the Naru-3 group significantly decreased (P<0.05). The apoptosis rate (11.400% ± 0.953% vs. 14.260% ± 1.105%) of the spinal cord in the Naru-3 group significantly decreased (P<0.05). Conclusions The Naru-3 solution can reduce the rate of spinal cord cell apoptosis after spinal cord injury, its mechanism may be related to the suppressed caspase-3 protein expression.
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Objective@#To observe the effect of the Mongolian medicine on the apoptosis after spinal cord injury.@*Methods@#A total of 12 rats were divided into the sham group, the model group, and the like-3 treatment groups (Naru-3 group), 4 in each group. A rat model of spinal cord injury (SCI) was established by modified Allen's method. After 72 h, we sacrificed the rats, separated spinal cord cells, which were filled with 5×105 shop in every hole, training for subsequent testing after 24 h. The relative expression of caspase-3 was detected by RT-QPCR and western blot, and apoptosis was analyzed by flow cytometry.@*Results@#Compared to the model group, the expression of caspase-3 mRNA (2.177 ± 0.084 vs. 3.287 ± 0.098) and caspase-3 protine (1.063 ± 0.157 vs. 2.180 ± 0.147) in the Naru-3 group significantly decreased (P<0.05). The apoptosis rate (11.400% ± 0.953% vs. 14.260% ± 1.105%) of the spinal cord in the Naru-3 group significantly decreased (P<0.05).@*Conclusions@#The Naru-3 solution can reduce the rate of spinal cord cell apoptosis after spinal cord injury, its mechanism may be related to the suppressed caspase-3 protein expression.
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Objective :To analyze therapeutic effect of Mongolian medicine Guanxinshutong (MM) capsule on restenosis af—ter stenting in patients with acute myocardial infarction (AMI).Methods : The 108 AMI patients ,who received stenting in our hospital from May 2014 to May 2016 ,were selected.The patients were equally divided into routine treatment group and MM group (received MM capsule based on routine treatment group ) ,therapeutic processes of both groups was one year .Serum levels of endothelin (ET)—1 ,nitric oxide (NO) and C reactive protein (CRP) before and one year after treat—ment ;recurrence rate of angina pectoris ,coronary restenosis rate and incidence rate of adverse cardiac events during one—year follow—up were observed and compared between two groups .Results : Recurrence rates of angina pectoris on six months and one year after treatment were significantly lower than that of three months after treatment (9.25%,5.56%vs.33.33%) in MM group (P＝0.002 ,0.001) ,and significantly lower than those of routine treatment group (25.93%, 22.22%) , P＝0.023 ,0.012. On one year after stenting ,coronary restenosis rate of MM group was significantly lower than that of routine treatment group (9.26% vs.33.33%) , P＝0.002. Compared with before treatment ,on one year af—ter treatment ,there were significant reductions in serum ET—1 and CRP levels ,and significant rise in NO level in two groups , P＜0.01 all ;compared with routine treatment group on one year after treatment ,there were significant reductions in serum levels of ET—1 [(125.09 ± 33.41) ng／L vs.(107.16 ± 34.26) ng／L] and CRP [(18.59 ± 4.63) mg／L vs.(7.23 ± 3.74) mg／L] ,and significant rise in NO level [ (6.81 ± 1.47) μmol／L vs.(11.45 ± 1.35) μmol／L] in MM group , P＜0.01 all.During one—year follow—up ,incidence rate of adverse cardiac events in MM group was significantly lower than that of routine treatment group (9.26% vs.25.93%) , P＝0.023. Conclusion : Mongolian medicine Guanxinshutong cap—sule can significantly prevent restenosis ,reduce recurrence of angina pectoris ,serum ET—1 and CRP levels and incidence of adverse cardiac events ,and improve prognosis in AMI patients after stenting .